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【High-throughput sequencing】 High-throughput sequencing

High throughput sequencing (High-throughput sequencing), also known as "next generation" sequencing, which can sequence hundreds of thousands to millions of DNA molecules in parallel.
【PCR Real-time fluorescence quantitative】 Real-time fluorescence quantitative PCR

Real-time fluorescence quantitative PCR (PCR) is a method of adding fluorescent groups to PCR reaction system and using fluorescence signal accumulation to real-time monitor the total amount of products after each (PCR) cycle of polymerase
【miRNA】 miRNA

The small RNA is the regulating RNA with molecule of 1928 nt, which mainly includes micro RNA (micro RNA,miRNA) and small interference RNA (short interfering RNA,siRNA).
【siRNA】 siRNA

SiRNA is a small RNA with molecule of ~ 21-25 nucleotides, which is produced by Dicera specific enzymeto double stranded RNA in the RNAaseⅢ family.
【SNP】 SNP

SNP, is called single nucleotide polymorphism, which mainly refers to the DNA sequence polymorphism caused by single nucleotide variation at the genomic level.
【Methylation detection】 Methylation detection

DNA methylation is refer to change 5’cytosineof CpG dinucleotide to methylcytosine through induced by DNA methyltransferase (DNMTs) , and is one of the earliest approaches.
【Site - directed mutagenesis】 Site - directed mutagenesis

Site-directed mutagenesis is the introduction of desired changes (usually changes in the favorable direction) into the desired DNA fragments (either genome or plasmid) by means of polymerase chain reaction (PCR),
【Vector construction】 vector construction

Vector construction is to transport the target DNA molecule to the recipient cells, so we must find a carrier that can enter the cell and load the foreign DNA fragment into the cell, and still copy it in the same way.
【Western Blot（WB）】 Western Blot

Western blotting is an important technique used in cell and molecular biology. By using a western blot, researchers are able to identify specific proteins from a complex mixture of proteins extracted from cells
【Autophagy overall solution】 Autophagy overall solution

Autophagy or autophagocytosis: also known as type II cell death, is a process in which cells degrade their damaged organelles and macromolecules by using lysosomes under the regulation of autophagy related gene (ATG).

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